(Vestsi Natsiyanalnai Akademii Navuk Belarusi)
SERIES OF BIOLOGICAL SCIENCES
Published by The Belaruskaya Navuka Publishing House, Minsk, Republic of Belarus
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Proceedings of the National Academy of Sciences of Belarus (Vestsi Natsiyanalnai Akademii Navuk Belarusi) SERIES OF BIOLOGICAL SCIENCES Published by The Belaruskaya Navuka Publishing House, Minsk, Republic of Belarus |
Summary: Information on comparative study of callus formation from different explants of potato is presented in article.
Rupasova Zh.A., Kukhareva L.V., Ignatenko V.A., Vasilevskaya T.I., Tytchyna I.N.
Characteristic peculiarities of seasonal accumulation of phenol compounds in plants of sweetweed (Althaea officinalis L.) when introduced in Belarus. pp. 10--14
Summary: The power of influence of weather conditions during vegetation period on the level of accumulation and character of seasonal dynamics of phenol acids, bioflavonoids and tannig substances in some organs of sweatweed when introduced in soil-climatic conditions of Belarus are shown for the first time.
Zavadskaya L.V., Khokhlov I.V.
The morphological changes of lilies (Lilium) after treatment by low intensity laser radiation. pp. 15--16
Summary: The data on influence of low intensity laser radiation on lilies development from radiated cloves is given. In the result of treatment the quantity of produced cloves and mass of free-year plants were increased.
Torchik V.I.
Characteristic peculiarities of wood plants growth and development in interior under moderate climate conditions. pp. 17--20
Summary: As an alternative for tropical and subtropical species for growing in interior representatives of the family Cupressaceae F. W. Neger are recommended as having broad natural areal, as well as species with narrow areal but growing in a wide range of edaphic conditions.
Prokhorov V.N., Laman N.A.
The spring barley (Hordeum vulgare L.) productivity prognosis by character of growth process intensity. pp. 21--29
Summary: In different soil and climate conditions were revealed dependence between accumulated biomass on the stage of 5 leaves (full tillering) and final seed productivity of spring barley. This dependence is described by linear equalization. The last allows to prognose the productivity of spring barley at the early stage of vegetation with high accuracy and correct the complex of agrotechnical methods to achieve the maximal productivity.
Mironenko A.V. †, Bushueva S.A., Domash V.I., Kandelinskaya O.L., Pelageichik T.Ya., Chekhova A.N., Uralskaya E.R., Ghabinsky V.N.
Physiological and biochemical actions of epibrassinolid on developing lupine (Lupinus) seeds. pp. 30--32
Summary: It has been studied epybrassinolide influence on metabolism of developing lupine seeds This phytohormone helps to accumulate the chlorophyll and nucleic acids accumulation, prolongates the life time of "protemaze--inhibitor" complex during the first 72 hours of developing After that time the dissociation and activity regeneration of its components was occur.
Ilchenko V.P., Trukhanovets N.L., Ruban V.V., Khotyleva L.V.
Structural changes of chloroplasts and mitocondria in fiber flax (Linum usitatissimum) in ontogenesis. pp. 33--41
Summary: Structural changes of chloroplasts and mitochondria were studied in fiber flax in ontogenesis. Chloroplasts of modem varieties were revealed to hahe more developed photosynthesizing system and its stable state during plant growth consistent with change in some traits defining an active state of chloroplasts. All the fiber flax genotypes studied had higher values for mitochondria membrane system at the early developmental stage.
Tarutina L.A., Poskannaya S.I., Mishin L.A., Kapusta I.Â., Khotyleva L.V.
The genetic components of variability and there connections with greenhouse tomato (Lycopersicum esculentum) geterosis. pp. 42--45
Summary: Productivity components defining heterosis in the F1 hybrids of greenhouse tomato response differently to an environmental change: the environment has a lesser effect on the average fruit weight and a greater effect on fruit weight and quantity per plant in early and total yield.
Guretskaya V.S., Gordey I.A.
The genotipical specificity of androgenesis for barley (H. vulgare L.) anther culture in vitro. pp. 46--49
Summary: For anther culture in vintro barley the factors and the values of influence on donor plants were optimized as well as the composition of culture media in respect of nutrition sources and biologically active combinations (BAC) for cultivation of anthers, calluses. Application of various BAC combinations: 2,4-D, 2,4,5-T, benzimidasole, ethrel, resulted in barley regenerants outcome increase up to 20.7%, green regenerants -- up to 16.7%. A gene of high androgenic ability was identified in barley (Igri variety), its genoplasm is recommended for introduction to hybridic plants of the 1 st generation.
Kulminskaya I.V., Guzyuk L.I., Shimko V.E., Khmyl T.O.
Genetic features of self-fertile alloplasmyc rye (Secale cereale). pp. 50--53
Summary: The article shows the possibility of producing self-fertile alloplasmic rye being of practical interest for its morphological and physiological traits and properties.
Petrovskaya O.B., Lyakhnovich G.V., Volotovsky I.D.
The phosphoinositidies phosphorilation in plasma membrane of etiolated oat seedlings (Avena savita L.). pp. 54--57
Summary: The article presents the results of the study on the phosphate (32P) incorporation into phosphoinositides and phosphatidic acid in plasma membrane (PM) isolated from etiolated oat seedlings. It was shown that PA, PI, PIP and PIP2 arc present in PM. 32P incorporation depends on Mg2+ concentration and pH, and has rapid kinetics from polyphosphoinositides PIP and PIP2 (t1/2 of 7--10 min at different pH range).
Ilkovets I.M., Sokolovsky S.G., Nait M.R., Volotovsky I.D.
The phytohormon control of Ca+2 concentration in plant cell protoplasts. pp. 58--62
Summary: The effect of indole-3-acetic acid, 1-naphtylacetic acid, gibberellic acid, abscisic acid, 24-epibrassinolide on the cytosolic free Ca2+ concentration ([Ca2+]cyt) in protoplasts of Nicotiana plumbaginifolia in Ca2+-deficient and Ca2+-conteined media was tested. It was observed that these phytohormons were able to increase [Ca2+]cyt up to 328 nM. The effect of auxins and epibrassinolide on [Ca2+]cyt was suppressed by verapamil. These data suggest that Ca2+cyt is a member of phytohormons transduction chain.
Shalygo N.V.
Accumulation of Í2Î2 in greening barley leaves (Hordeum vulgaris L.) under condition of porphyrinogenesis induced by Cs+ cations. pp. 63--67
Summary: Accumulation of H2O2, change of the catalase (Cat) and ascorbate peroxidase (APX) activity in greening barley leaves containing uroporphyrinogen III (UROgen), uroporphyrin III (URO) and the inactive protochlorophyllide (Pchlide) have been investigated. The Cs+ cations were used as inducers of porphyrinogenesis. It was demonstrated that the H2O2 amount and the Cat activity in leaves accumulating UROgen did not change and the APX activity increased in comparition with the control. The H2O2 pool increased in leaves accumulating URO and Pchlide. Stimulation of only the APX activity was observed at these conditions. Cat was more sensitive to photodynamic effect (PDE) induced by URO and Pchlide. So, its activity decreased after beginning of PDE after that the APX activity begin to decrease as PDE enhanced. The correlation between the enzyme activity and reducing of the Cat and APX protein amounts in immunobloting was shown. Peculiarities of H2O2 accumulation, change the Cat and APX activity by inducing of Cs+ porphyrinogenesis are discussed.
Borod'ko N.V., Romanovskaya T.V., Kolomiets E.I., Bayeva S.V., Lobanok A.G.
The sporulating bacteria of genus Bacillus -- antagonists of phytopathogenic microflora. pp. 68--72
Summary: Research was carried out on isolation, selection and identification of sporulating bacteria of genus Bacillus - antagonists of phytopathogenic microflora. Strain Bacillus pumilus 203 displaying high antagonistic activity towards a broad spectrum of phytopathogenic microorganisms was shown to be of a greatest interest for biological control. Composition of nutrient media for submerged culture of isolated variant was optimized allowing to raise its antifungal and antibacterial activity by 30 and 54%, respectively, in competition with the control (Meynell medium).
Astapovich N.A., Shcherba V.V., Osadchaya O.V.
The flint influence in liquid media on melanin pigment formation in some species of micro- and macromyceties. pp. 73--75
Summary: Addition of flint water extract (4--12 % v/v) to liquid media for fungi cultivation favours the activation of phenoloxidase complex ferments. It increase the melanin production in 1.2--1.9 times.
Goncharova I.A., Sokolova T.V., Babitskaya V.G., Tomson A.E., Shcherba V.V., Pekhtereva V.S.
Heavy metals sorption by Melanin-Synthesizing Basidial Fungus Phellinus robustus. pp. 76--78
Summary: Heavy metals sorption ability of tinder fungus Phellinus robustus has been studied. Sorption capacity of the pigmented mycelium or the pure fungal melanin was higher than one of the light biomass. IR spectra comparative analysis of the melanin and the melanin-copper complex showed that copper ions were bonded with carboxylic groups. The order of binding affinity of the pigmented fungal biomass for heavy metals was: Fe2+ > Pb2+ > Cu2+ > Zn2+ > Cr3+ > Ni2+.
Safronova G.V., Mokhova S.V.
The indole-3-acetic acid synthesis by nitrogen fixing enteric bacteria. pp. 79--81
Summary: It is shown that the nitrogen fixing enteric bacteria (Klebsiella planticola 5, Enterobactor sp. 11 and association Citrobacter freundii la + Alcaligenes faecalis 1b) produce indole-3-acctic acid (IAA) in clean cultures when media were supplemented with L-tryptophan and increase the content of IAA in inoculated plants.
Zalashko M.V., Kartel A.N., Grushenko M.M.
The fermentation of whey lactose by the yeast Kluyvero-myces marxianus. pp. 82--85
Summary: The fermentatiom of whey lactose by the yeast Kluyveromyces marxianus in the following conditions: aerobic, anaerobic with the stirring and anaerobic without stirring has been studied. It is shown that the most favourable for the fermentation of concentrated whey (the contend of lactose 11--13%) are anaerobic conditions with the continuous stirring of substrate.
Antonenko A.N., Lobanok L.M.
Functional response of white rats isolated heart to the stimulation of andrenergic receptors affer the g-irradiation in low doses. pp. 86--89
Summary: The study of heart isolated by Langendorf's method has shown that the acute g-irradiation of rats in 1.0 Gy dose (9 • 10-4 Gy/s) causes the decrease of contraction ability and relaxation of myocardium as well as of the functional response of heart to the stimulation of a- and b-adrenergic receptors.
Mazhul L.M., Volykhina V.E., Bagel I.M., Gatsko G.G.
The lipid peroxidation system in the blood of the experimental rats under external and incorporated irradiation. pp. 90--92
Summary: The state of lipid peroxidation in the blood of 6--7 month old male rats inder the combined ray action (chronic g-irradiation at the dose of 1 Gy and the incorporation of 137Cs at the doses of 160 and 320 kBq) was studied. It was shown that the large dose of incorporated 137Cs in early terms renders the stimulating influence on the antioxidant system of organism, possibly, promoting thus the support of the invariable level of lipid peroxidation products.
Petushok N.E., Lashak L.K., Trebukhina R.V.
The effects of the 3-fold g-irradiation on the glutathion system of blood, brain and liver of white rats. pp. 93--95
Summary: The effects of the 3-fold g-irradiation (total dose 0.75 Gy) on the glutathione system at different periods after exposure in the blood, brain and liver of rats were studied. The phase changes were found. The most pronounced they were in the blood. Activation of the system take plase at early terms (I hour) after irradiation, then it was exhausted. In the brain and liver the system was more stable.
Emel'yanova A.A., Zhukova N.D.
The participation of andrenergic and serotoninergic substances in condition of gastric mucosa ulcer formation of white rats. pp. 96--99
Summary: Changes in the fine structure of neurons and their processes of the dorsal motor nucleus of the vagus nerve and structural alterations of adrenergic terminals and serotonin-containing cells of the gastric mucosa of rats in conditions of experimental ulcer genesis were found. Possible mechanisms underlying these changes are discussed.
Borisov O.L.
Reflex responses of sympathetic efferent fibers of somatic and visceral nerves of white rats. pp. 100--103
Summary: Responses of n. saphenus and superior mesenteric nerves of the ileum, induced by electrical stimulation of ileocecal nerves, were studied in order to reveal the patterns of both reflex responses. Comparative analysis of the responses to impulses from mesenteric C-afferent showed that the responses in n. saphenus had a longer latency (480 ± 3) and much a shorter duration (190 ± 3). Tetanic stimulation induced complete arrest of tonic discharge in cutaneous nerve fibres and vigorous efferent activity in mesenteric nerves. The study indicates considerable differences in the mechanisms of centra] switching of excitation of visceral afferent fibres to sympathetic efferent neurons which control the activity of abdominal organs and skin blood flow.
Mokhort T.V., Goranov V.A., Mitskevich P.B., Zaborovskaya Z.V., Murzenok P.P.
The influence of interleykin-1 and streptozotocin on survival of Langergals beta-cells in vitro. pp. 104--106
Summary: The influence of combination streptozotocin and interleukin-1 on survival of beta-cells in vitro was investigated. Addition of interleukin-1 and nontoxic concentration of streptozotocin essentially potentiations the damage effect.
Davydovskii A.G.
Influence of E. coli lipopolysaccharide and phenobarbital on lipid peroxidation and antioxidant defense in liver thymus of white rats. pp. 107--109
Summary: Effects of LPS E. coli on lipid peroxidation and antioxidant defense ensymes state in liver and thymus after previous induction of cytochrome P450-dependent monooxyganase system by phenobarbital (PB) was investigated. Possibly, PB as well as previous induction of drug metabolizing enzymes system increases cross-resistance to damaging environmental factors.
Pikulik M.M., Kulesh V.F., Alekhnovich A.V., Pareiko O.V.
Ecological consequences of appearance of North American crayfish species (Orconectes limosus Raf.) in water ecosystems of Belarus. pp. 110--112
Summary: The fact of appearance of North American crayfish species (Orconectes limosus Raf.) in Belarus (the river Shiyamitsa, a tributary of the Neman, June of 1997) is reported. The history of its apperance and distribution on the European continent and the biological features and morphological distinctions are given briefly. Ecological consequences of interrelations of the new species to the native fauna as well as possibilities and an expedience of the commercial use are planned to be studied.
Shlyakhtenok A.S., Agunovich R.G.
The variability of the spot's shape on the clypeus of 3 species of social wasps Vespula Thoms. (Hymenoptera, Vespidae). pp. 113--118
Summary: The variability of the spot's shape on the clypeus of 3 species of social wasps -- Vespula rufa (L), V. vulgaris (L.), V. germanica (F.), collected by Malaise traps and entomological nets are analysed. For every species the number of spot's types and their distribution among castes are presented. The variability of the spot's types ratio during a season, a variability in different biotopes, nests on the North and the South of Belarus are examined.
Rizevsky V.K., Plyuta M.V., Ermolaev V.V.
The morphological characteristic of Amur sleeper Perccottus glehni Dybowski from Minsk water reservoir system. pp. 119--121
Summary: The biometrical features description of Amur sleeper Perecottus glehni Dybowski for the first time for Belarus has been given. The single sex distinction -- the height of the second dorsal fin was revealed. The reliable differences between 7 biometrical features of Amur sleepers inhabited the Minsk water reservoir system and the Hunk Lake were registered.
Kunitsky D.F., Plyuta M.V.
Stone moroco (Pseudorasbora parva) -- new species of Belarus ichtyofauna. pp. 122--125
Summary: The morphological description of new species of Belarus ichtyofauna stone moroco (Pseudorasbora parva (Schlegel)) is given. The reliable distinctions on 17 morphometric features of stone moroco from the Pteech river (Pripyat river basin) and from Armenian reservoirs are recorded.
Summary: Clinical and experimental data of the literature about the influence of chronic alcohol intoxication on the lipid spectrum (fatty acid, cholesterm, triacilglycerines, phospho-, glycolipids and their fractional composition) of the tissues (blood, liver, brain) have been analysed. Considerable changes in lipid metabolism under chronic alcoholization have been revealed. Some mechanisms of these disturbances are discussed.
Summary: Substrate specificity of rat kidney and liver sorbitoldehydrogenases was investigated. The obtained kinetic parameters were similar for enzymes from both tissues. It suggests that liver does't contain specific isoenzyme sorbitoldehydrogenase.
Vakhnii N.A., Veremchuk O.N., Zhukovsky A.T., Kovalevich N.N.
About rare of vessel plants from Brest region. pp. 136--137
Summary: In Belarus new locations of 13 rare of plants were revealed. Among them 4 species are indicated for Brest region for the first time and 1 -- for the republic.
Chumakov L.S., Khatskevich T.M., Shcherbakova T.M.
Over-all investigation of recreated forests of summer camps. p. 138
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