THE NATIONAL ACADEMY OF SCIENCES OF BELARUS


Proceedings of the National Academy of Sciences of Belarus
(Vestsi Natsiyanalnai Akademii Navuk Belarusi)
SERIES OF BIOLOGICAL SCIENCES

Published by The Belaruskaya Navuka Publishing House, Minsk, Republic of Belarus

Number 3, 2000

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CONTENTS


ARTICLES

Reshetnikov V.N., Kaler V.L., Bulko O.P., Bykova L.N.
Morphological and growth parameters of Scenedesmus acutus culture under the influence of consument-factor excreted by Daphnia magna. pp. 5--7

Summary: Scenedesmus culture growth parameters are shown to be not correlated to the share of cells in colony state. The culture growth dynamics is discerning from morphometric changes dynamics. Hence, inhibition of scenedesmus culture growth induced by daphnia excreted consument-factor can't be explained solely by decrease of specific nutrition surface caused by colonies formation.

Reshetnikov V.N., Kaler V.L., Bulko O.P., Bykova L.N.
Joint action of culture media and Daphnia magna excerted consument-factor on Scenedesmus acutus culture morphology and growth. pp. 8--10

Summary: Joint action of growth medium concentration and Daphnia magna excreted biotic factor concentration on Scenedesmus acutus culture morphology and growth rate was studied in 2 factor on 2 levels experimental design. Response surface plotting showed the consuurent factor to induce coenobia formation in diluted medium and to inhibit this process in concentrated nutrient medium. Growth medium elevation activated coenobia formation on low and suppresed it on high levels of consument factor. Culture growth parameters gave similar responses in the beginning of cultivation, but after 3 days of cultivation in diluted medium the inductive activity of consument factor was not shown on all the response variables studied. Scenedesmus culture in diluted medium is supposed to eat out some medium component needed to induce morphometric and growth parameter changes by daphnia excreted factor.

Rupasova Zh.A., Kutas E.N., Zlotnikov A.K., Ignatenko V.A., Vasilevskaya T.I., Varavina N.P., Rudakovskaya R.N., Matjushevskaya E.N.
Influence of propagation techniques on chemical composition of rhododendron (Rhododendron L.) leaves. pp. 11--16

Summary: Interspecific differences in biochemical composition of assimilatory organs of japanese and Smirnov Rhododendron propagated by generative and meristem techniques have been determined for the first time.

Glushakova N.M.
Comparative peculiarities of gerbera (Gerbera jamesonii Bolus) species growing in the Central Botanic Garden of the National Academy of Sciences of Belarus. pp. 17--20

Summary: Comparative characteristics of 14 species of gerbera growing in the Central Botanical Garden of the National Academy of Sciences of Belarus are given in the article. They include: holland selection -- 5 species, latvian -- 3 species, belarussian -- 6 species. To enrich the genofund of pot culture of gerbera and to get resistant, productive and highly decorative species of belarussian selection the investigation by introduction and selection is recommended.

Shukanov V.P., Polyanskaya S.N.
Steroid glycosides as initial growth regulators in cultivated cereals. pp. 21--23

Summary: A comparative effect of endogenous steroid glycosides of faro- and spirostanol series on growth of wheat- and triticale seedlings was studied under laboratory conditions. High wheat resistance and low triticale one to steroid glycoside treatment were revealed. If the latter primarily stimulated growth of wheat seedlings at the concentrations of 0.1--50 mg/l, growth of triticale seedlings was suppressed. Cereals coleoptiles turned out more responsive to steroid glycoside treatment for both general activity and selectivity of the effect. Steroid glycosides proved to be pronounced growth regulators of auxin type during the initial growth of cereals.

Zankevich N.P., Volynets A.P.
Antifungal activity of flavonoid glycosides of rye. pp. 24--26

Summary: Antifugal activity of flavonoid glycosides of rye was studied for the first time. It was established, that flavonoid glycosides in minimum concentration already inhibit growth of spors of rye rust fungi, Flavonoid glycosides begin to defend plant from pathogen on the surface of vegetative organs, but the main defence with flavonoid take place in the localizating zone of rust fungi into the tissue of plant.

Kordijako N.G.
Growth velocity and morphological peculiarities in culture of the polyporus fungi under different temperature. pp. 27--29

Summary: The article presents the materials of morphological peculiarities in culture and speed of the polyporus fungi growth, which are predominant in the parks and gardens.

Korinyak S.I.
Pathogen fungi on Bergenia paciflca Kom. under introduction in Belarus. pp. 30--31

Summary: The identification of pathogen fungi on Bergenia pacifica Kom. has been done. The 4 species of fungi: Ramularia bergeniae Vassjag., Ramularia saxifragae Syd., Alternaria alternata Fr., Ascochyta philadelphi.Sacc. have been isolated. For determination of lesion rate the international nine mark scale has been used. Altemaria altemata (Fr.) appeared to be the most harmful among pathogens.

Urajeva M.B., Laman N.A.
Synchronization of leaf appearance on leading shoot of barley (Hordeum vulgaris L.) and qualitative changes in apical meristem. pp. 32--34

Summary: The time of apex transition to generative morphogenesis was shown to be easily determined by the external morphological trait - the number of fully expanded leaves which for each particular variety is calculated by subtracting the figure 5 from the total number of leaves formed on leading shoot.

Yarchanka Ya.A.
Biodiversity of corticioid fungi (Corticiaceae S. L., Basidiomycetes) in Belarus. pp. 35--37

Summary: The number of species of corticioid fungi in Belarus were counted. The revision was made on the collection material (herbarium MSK, Fungi) and literature sourses. The nomenclatural questions was solved by means of database CORTBASE 1.2, compiled by E.Parmasto. In the mycobiota there are 180 species (holomorphs) of corticioid fungi that are confirmed by the herbarium was registered. The species belonged to 68 genera. Nine species are known only from literature. 38 species was mentioned in the article for the first time in Belarus.

Budaj S.I.
Germinating capacity and morphophysiological peculiarities of developing carrot plants under treatment by growth regulators. pp. 38--41

Summary: It has been shown that pretreatment of carrot seeds by epybrassinolide, diphenylurea, naphthylacetic acid and succinic increases the germinating force, ability and simultaneity of shoots. The grown-up plants possessed more producing ability due to the powerful leaf organs and root-crops with decreased nitrate content.

Sen L.A., Volodin V.G., Bondarevich E.B.
Sisterly chromatide exchanges of chromosomes of some unstable wheat genotypes. pp. 42--45

Summary: The study of unstable mutant genotypes in spring and winter wheat has shown that their SCE frequency for such parameters as the number of chromosomes with exchanges, the SCE number per chromosome and metaphase plate is higher then in the initial forms and constant mutants. In F1 hybrids of unstable genotypes with the initial varieties the SCE frequency is intermediate between parental forms.

Gancharova R.I., Smolich I.I., Nikitchanka N.V., Kapitanava N.P.
Adaptive response of bank vole (Clethrionomys glareolus, Schreber) whose population has been lived in radioactively contaminated areas. pp. 46--52

Summary: According to data established in 1996, the frequencies of micronucleated erythrocytes in bone marrow and peripheral blood of bank vole (Clethrionomys glareolus, Schreber), whose populations have been lived in contaminated areas since 1986 depend on chronic low intensity irradiation (dose rates in the range from 2.4 to 41.3 mGy/day). The forms of these dose-effect relationships for the observed cytogerietic injuries are nonlinear and better described by the parabola equations. It is found that inducible repair system "adaptive response" functionates quite well within 21st--24th bank vole generations after the Chernobyl accident.

Pavlyuchuk N.V., Akhromenka A.D., Palilova A.N.
Feature of resistance instability to X-virus of potato (Solanum tuberosum L.) clones subjected to multistep selection. pp. 53--55

Summary: The level of resistance instability to X-virus was studied in potato clones that were subjected to multistep selection. Possible genetic causes of the variability observed for this character being discussed. Relationship between the PVX resistance behaviour in vegetative clone generations and the genotype of the original variety and parental plant in each clone was revealed. The vegetative clone progeny with variety genomes, characterized by minor heterogeneity in resistance, maintains the resistance degree at the level of the original plant. On the contrary, the clones with variety genomes characterized by great heterogeneity in PVX resistance produced segregation for this character in the progeny. The observed instability of resistance to X-virus can be associated with a number of factors: with somatic mutations and somatic crossing-over in cells and tissues, whose frequency increases at vegetative propagation of clones.

Belko N.B., Gordei I.A., Khokhlova S.A., Lyusikov O.M.
Morphobiological features of rye-triticale F1 amphihaploids. pp. 56--59

Summary: Manifestation of agronomic characters and properties was studied in rye-triticale F1 amphihaploids. A positive effect of rye cytoplasm on viability, terms of plant heading and flowering, as well as on formation of fertile pollen was shown.

Voitovich A.M.
Frequency of micronuclei in erythrocytes and disturbance of differentiation process in erython of brown frog under chronic radiation. pp. 60--63

Summary: The micronucleus assay was used as a fast test for ecologogenetic monitoring in radiocontaminated areas. The occurence frequency of erytrocytes with micronuclei in studied animals is 9--30 times as high as in the control ones and correlated with cytogenetic damages in bone marrow cells. The deviations in amphybian erythron were also observed under radiocontamination.

Kilchevsky A.V., Babak O.G.
Peculiarities of dominance level and heterosis manifesting at traits characterizing photosynthetic efficiency of tomato under various nutrient environment. pp. 64--67

Summary: Diallel analysis of the traits characterizing photosynthetic efficiency of tomato was conducted to evaluate their variability and peculiarities of level of dominance and heterosis manifestation depending on farming conditions. This investigation demonstrates the influence of weather conditions and level of mineral nurishment on the following traits: Net Assimilation Rate, Relative Growth Rate, Leaf Area Ratio. More frequent geterosis manifestation on the traits under study was defined in unfavorable environmental conditions.

Blotskaya Zh.V., Rusetsky N.V.
Interferention of PVM, PVY, PVX in potato (Solanum tuberosum L.) plants under complex estimation to immunity. pp. 68--70

Summary: Results of research on evaluation of PVY, PVX, PVM interaction in potato plants with different degree of resistance are presented. Positive and negative interferention of PVX, PVM, PVY was found in succesive and mixed inoculations of potato and tomato plants. Trials of synergism and antagonism between viruses depend on resistance of host-plant and combination of viruses in inoculume and succession of their application. In detection of complex resistance to PVX+PVM+PVY it is not recomended to aplly mixed inoculation of potato plants to avoid interferention between them.

Fradkin L.I., Kolyago V.M., Domanskaya I.N., Domanskij V.P.
Fractionation of the plastids from green and greening plants treated with digitonin using the gel-electrophoretic method. pp. 71--76

Summary: New procedure is described for the method of gel-electrophoresis of the plastids treated with digitonin (Dig-PAGE) put forward earlier by authors. The effect of some treatments of the plastids on Dig-PAGE results is shown. Dig-PAGE provides a means of separating of the intramembrane particles and a portion of plastid lipids, perhaps of lipids from the membrane bilayer. It is shown, that during greening of the etiolated barley leaves intermediary forms of the intramembrane particles initially emerge and they gradually transform into the mature intramembrane particles inherent in the green barley leaves.

Melnikov S.S., Manankina E.E., Budakova E.A.
Stimulin 5 doesn't stimulates the alga growth. pp. 77--79

Summary: The cells of Euglena gracilis, Chlorella kessleri, Scenedesmus acutus, Dunaliella salina, Spirulina platensis were grown in the mediums with new plant growth regulator (stimulin 5, 10-6M -- 10-18M). No changes in the productivity and contents of pigmtnts were found.

Emelyanova V.P., Baranova L.A., Volotovsky I.D.
Influence of products of phosphoinositides hydrolysis on ATP-dependent Ca27 accumulation by retinal rod organelles. pp. 80--83

Summary: The influence of IŠ3 and factors, stimulating the metabolism of phosphoinositides, on the ATP-dependent Ca2+-uptake in vesicular structures of the retinal rod (rod outer segments, microsomes and mitochondria) was studied. It was determined that the Ca2+-uptake efficiency in outer segments, microsomes and mitochondria depends on the presence of ATP in the medium. However, the products of the hydrolysis of the membrane polyphosphoinositides influence this prosess only in the retinal microsomes, that is probably related to the modulation Ca2+-pump on endoplasmic reticulum.

Zajtseva E.M.
Enzyme-substrate interactions and internal dynamic of glycogen phosphorylase b. pp. 84--86

Summary: Using room temperature tryptophan phosphorescence technique, the effect of enzyme-substrate interactions has been studied with respect to glycogen phosphorylase b internal dynamics. It has been established that binding of substrate (glucose-1-phosphate) to the enzyme active site results in functionally significant changes in the enzyme millisecomds internal dynamics. It has been shown that the substrate binding affects both conformation of the enzyme and its large-scale internal dynamics; the observed restrictions of structural fluctuations are connected with cooperative subunits interactions.

Popov E.G.
The influence of external g-irradiation at low doses on the specific binding for androgen receptor by nucleus of liver and prostate gland cells. pp. 87--90

Summary: The phasic's changes of the nuclear specific binding for androgen-receptor complexes subsequently caused by the prolonged external g-irradiation (5.83 • 10-7 Gy/s) at low doses (0.1--1.0 Gy) were observed in a male albino rats liver and ventral prostate cells during 365-days period after termination of the factor and were correlated with the permanent secretory increase of testosterone in blood serum. The data obtained suggest the involvement of the processes in the development of male reproductive disfunctions after the low doses exposition.

Antonenko A. N., Gorokh G.A., Lobanok L.M.
The nearest and the remote safest of acute g-irradiation on the biomechanical function of heart, its adrenergic regulation at hypothyrodism. pp. 91--95

Summary: The study of isolated Langendorf-perfused heart has shown that the acute g-irradiation of rats in 1.0 Gy dose (9 • 10-4 Gy/s) causes the decrease of contraction ability, myocardium relaxation, and reaction of heart to the stimulation of a- and b-adrenergic receptors. The irradiation of hypothyroid animals leads to more expressed and long changes of functional state of heart and modifies its adrenergic regulation.

Lobanok L.M., Suvorova T.A.
The influence of NO-synthase on the functional state of the isolated rat heart under hypoxia and reoxygenation. pp. 96--98

Summary: The investigation of the isolated Langendorff-perfused rat heart has shown that the hypoxia causes coronary vasodilation and attenuation in heart contractility. The blockade of NO synthesis decreases the hypoxic vasorelaxation of coronary vessels and leads to impairment of the biomechanical heart function, decreases the free-radical injury of myocardial and endothelial cells during reoxygenation.

Kandybo T.S., Rozhnova L.E.
Sustained stress state condition investigations of rats. pp. 99--101

Summary: Dysregulation of neuronal activity in the ventrolateral medulla, induced by application of glutamate to the RVLM, led to an increased perception of a negative stimulus as evident from the behavioral strategy of rats, which may indicate sustained stress state resulting in and triggered by the generation of negative emotions.

Arinchin N.I. †, Efimova L.A.
The role of the mycropumps of skeleton muscles and respirative Henderson pumps in peripheral hemodynemic. pp. 102--104

Summary: Additional data were obtained suggesting a significant role of the micropumps of skeletal muscles (along with other extracardial factors of circulation) in the regulation of organ blood flow and its rate in the nutritive pathway.

Semenenya I.N.
The neuron population activity of the solitary tract nucleus under condition of animal cooling with subfebrility. pp. 105--108

Summary: An electrophysiological investigation of the hypothalamus medial preoptic area activity and the solitary tract nucleus in guinea pigs with Freund's complete adjuvant-induced subfebrility in conditions of cooling of the animals revealed a shift of the threshold of thermally induced changes in the multicellular electrical activity of the solitary tract nucleus towards lower temperatures as compared with control animals.

Gorodetskaya I.V.
The dependence of heat adaptation efficiency from the thyroidea status of organism. pp. 109--113

Summary: Depression of glandula thyroidea thyroid function during late period of heat trainings removes realization of heat adaptation protective effects, that take place in intact animals -- limitation of hyperthermia, gastric mucosa ulceration, changes of adrenal glands and spleen relative mass, of gormone and metabolites blood concentration, provoked by heat stress. The use of thyroid hormones at small doses during this period, on the contrary, promotes to formation of effective heat adaptation and what's more the adaptation price in this case is less than in intact rats. Therefore, thyroid hormones are of importance in heat adaptation development.

Stepanov L.V.
Immunocitogenetic analysis of organism conditions of the hosts Glardiasis. pp. 114--116

Summary: It was established that subclinical form of Giardiasis is characterized by the expressed changes of immunological and immunocitogenetical indicators. It was expressed by the reduction of general numbers of B- and T-lymphocytes and also by a number of cells restriction without and with the two associations of acrocentric chromosomes on the background of increasing of the Th, IgG and E contents and elevation of the average number of the associations to a cell. The therapy of Giardiasis is accompanied by the tendency to preserving the established disturbances disappearing during the recovery.

Giginyak Yu.G., Vlasov B.P., Generalova Yu.I.
Evaluation of the ecological conditions of freshwater crayfish (Decapoda) inlakes of Belarus. pp. 117--121

Summary: Data on ecological situation in 650 Belarusian lakes have been analyzed. Their conformity to environmental requirements of freshwater crayfish (Decapoda) is shown. About 300 lakes correspond to these conditions taking into account the level of the vegetation development, the maximal and average depths, pH. Size structures of crayfish egg-bearing females are described. The size range of mature females of crustaceans from different climate zones is presented.

Razlutsky V.I.
Comparison of birth estimation precision in population of Cladocera by the standard and modified methods. pp. 122--125

Summary: If the egg ratio is calculated in view of egg age distribution, the birth rate can be estimated at short sampling intervals (less than egg stage duration) by modified Paloheimo's formula. Computer simulations have shown that this formula evaluates birth rate with the relative error of 60% and usually overestimates its values. Birth rate is estimated in 3.8 times more precisely (relative error of 16%) by the modified method than the standard one.

Maksimova S.L., Gurin V.M., Blinov V.V.
The structure of the soil invertebrates population in the biogeocenosis of the bank's river Ptich. pp. 126--130

Summary: The structure of soil invertebrates population was investigated in the biogeocenoses of the bank's river Ptich during 1998.

Galkovskaya G.A., Mityanina I.F., Zarubov A.I., Gorelysheva Z.I.
The influence of cuitural conditions of plankton crayfish Daphnia magna Straus on the growth rate and the volume of alga individuals of Scenedesmus acutus Meyen. pp. 131--133

Summary: The alga culture age and mineral nutrition concentration in the medium determine the alga Scenedesmus acutus Meyen response to addition of cultural medium of Daphnia magna Straus revealed by algae parameters such as growth rate and individual volume.

Kulak A.V.
New and rare species of noctuids (Lepidoptera, Noctuidae) of Belarus. pp. 134--136

Summary: The article cited the information concerning 14 rare species of Noctuidae. 6 of them (Heliothis ononis, Panemeria tenebrata, Agrochola litura, Mesapamea didyma, Archanara dissoluta, Cryptocala chardinyi) were discovered on the territory of the Republic for the first time. Their terms of the fly separate phases, the fodder plants caterpillar and also distribution in Belarus and in the neighbouring States are specified.

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SCIENTISTS OF BELARUS

Reshetnikov V.N., Sidorovich Ye.A.
Nikolai Vladislavovich Smolskij (To the 95th Anniversary). pp. 137--139
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Antonov I.P., Bekish O.-Ya.L., Votyakov V.I., et al.
Georgy Ivanovich Sidorenko (To the 75th Anniversary). pp. 140--141
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Sushchenya L.M., Octapenya A.P., Pikulik M.M., et al.
In memory of Nina Nikolaevna Khmeleva. pp. 142--144
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